The laser scanning confocal microscope facility of the Cannon Research Building includes a Leica DMIBE Inverted Microscope with 5X, 10X, 20X, 40X, 63X and 100X objectives coupled to a Leica TCS SP: Confocal Imaging Spectrophotometer. Four lasers are used in conjunction with this system and consist of a HeNe laser (633nm), a Krypton laser (561 nM), an argon laser (488 nm) and a Spectra Physics Millenia V pump/Tsunami Ti/Sapphire dual photon laser. The four-laser system provides capabilities to image a wide variety of fluorescently labeled cells (both fixed and viable cells). In addition, the dual photon laser system provides the ability to image live fluorescently tagged cells and deeper imaging of larger sections and materials. The 3D rendering packages provide users the ability to evaluate intra- and intercellular interactions and perform basic quantification analysis of the captured images.

Richelle Hemendinger, PhD manages the core facility. Assistance with project design, training on the instrument and technical support (including imaging of samples) is provided at an hourly rate to all users of this facility. An appointment is required for the use of this instrument. Please contact the facility for current rates, scheduling and instrument availability.

Contact Information

Email: CHSConfocalMicroscopyFacility@carolinas.org
Richelle Hemendinger, PhD
Phone: 704-355-9786